FISH Technology

Inhaltsverzeichnis

List of Contents.- The Standard FISH Procedure - an Introduction.- 1 FISH Techniques, FISH Probes and Their Applications in Medicine and Biology - An Overview.- 2 Microscopy and Imaging Systems.- FISH on Native, Human Tissues.- 3 FISH on Chromosome Preparations of Peripheral Blood.- 4 FISH on Uncultured Blood or Bone Marrow.- 5 FISH on Blood or Bone Marrow Smears.- 6 Epithelial Cells from Buccal Smears and Urine.- 7 FISH on Hair Root Cells.- 8 Amniotic Fluid Cells - Uncultured.- 9 Human Sperm Cells.- FISH on Archival, Human Tissues.- 10 Archival Cytogenetic Slides.- 11 Formalin-Fixed and Paraffin-Embedded Tissue Sections.- 12 Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue.- 13 Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue.- 14 Nucleus Extraction from Cryofixed Tissue.- 15 Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells.- 16 Comparative Genomic Hybridization.- Special FISH Techniques (Fiber-FISH, PNA-FISH, Co-FISH, NU-FISH).- 17 High Resolution FISH of Stretched Chromosome Fibers.- 18 Quantitative DNA Fiber Mapping.- 19 PNA-FISH.- 20 Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH.- 21 The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH).- 22 DNA Breakage Detection-FISH (DBD-FISH).- 23 Detection of Radiosensitivity by Means of Three-Color FISH.- 24 Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T.- FISH in Zoology and Virology.- 25 Zoo-FISH.- 26 FISH on Insect Cells Transfected with Heterologous DNA.- 27 Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells.- 28 In Situ Detection of Viral Nucleic Acids by Chemiluminescence.- Multicolor FISH Applications.- 29 Spectral Imaging Analysis of Metaphase and Interphase Cells.- 30 Microdissection of Chromosomes and Reverse FISH.- 31 Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements.- 32 Centromere-Specific Multicolor-FISH (cenM-FISH).- 33 Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material.- 34 Simultaneous Fluorescence Immunophenotyping and FISH (FICTION).- Not FISH but Almost.- 35 PRINS: Hybridization and Primed in Situ Labeling in One Step.- 36 Microarrays and DNA-Chips in the Analysis of Genetic Abberrations.

FISH Technology

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Beschreibung

Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.

From the reviews:

"Ever wondered how to catch your FISH ... in the dark pools of cellular biology? Then ... FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) ... . Every chapter is illustrated by several schematic drawings ... . The manual is completed by a subject index ... . The book is certainly clear ... providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

Details

Einband

Taschenbuch

Erscheinungsdatum

10.11.2012

Herausgeber

Bernd W. Rautenstrauss + weitere

Verlag

Springer Berlin

Seitenzahl

494

Maße (L/B/H)

24,2/19,3/2,7 cm

Beschreibung

Details

Einband

Taschenbuch

Erscheinungsdatum

10.11.2012

Herausgeber

Verlag

Springer Berlin

Seitenzahl

494

Maße (L/B/H)

24,2/19,3/2,7 cm

Gewicht

977 g

Auflage

2002

Reihe

Springer Lab Manual

Sprache

Englisch

ISBN

978-3-642-47739-3

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  • FISH Technology
  • List of Contents.- The Standard FISH Procedure - an Introduction.- 1 FISH Techniques, FISH Probes and Their Applications in Medicine and Biology - An Overview.- 2 Microscopy and Imaging Systems.- FISH on Native, Human Tissues.- 3 FISH on Chromosome Preparations of Peripheral Blood.- 4 FISH on Uncultured Blood or Bone Marrow.- 5 FISH on Blood or Bone Marrow Smears.- 6 Epithelial Cells from Buccal Smears and Urine.- 7 FISH on Hair Root Cells.- 8 Amniotic Fluid Cells - Uncultured.- 9 Human Sperm Cells.- FISH on Archival, Human Tissues.- 10 Archival Cytogenetic Slides.- 11 Formalin-Fixed and Paraffin-Embedded Tissue Sections.- 12 Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue.- 13 Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue.- 14 Nucleus Extraction from Cryofixed Tissue.- 15 Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells.- 16 Comparative Genomic Hybridization.- Special FISH Techniques (Fiber-FISH, PNA-FISH, Co-FISH, NU-FISH).- 17 High Resolution FISH of Stretched Chromosome Fibers.- 18 Quantitative DNA Fiber Mapping.- 19 PNA-FISH.- 20 Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH.- 21 The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH).- 22 DNA Breakage Detection-FISH (DBD-FISH).- 23 Detection of Radiosensitivity by Means of Three-Color FISH.- 24 Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T.- FISH in Zoology and Virology.- 25 Zoo-FISH.- 26 FISH on Insect Cells Transfected with Heterologous DNA.- 27 Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells.- 28 In Situ Detection of Viral Nucleic Acids by Chemiluminescence.- Multicolor FISH Applications.- 29 Spectral Imaging Analysis of Metaphase and Interphase Cells.- 30 Microdissection of Chromosomes and Reverse FISH.- 31 Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements.- 32 Centromere-Specific Multicolor-FISH (cenM-FISH).- 33 Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material.- 34 Simultaneous Fluorescence Immunophenotyping and FISH (FICTION).- Not FISH but Almost.- 35 PRINS: Hybridization and Primed in Situ Labeling in One Step.- 36 Microarrays and DNA-Chips in the Analysis of Genetic Abberrations.