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Fluorescence in situ Hybridization (FISH) Protocols and Applications

Aus der Reihe Methods in Molecular Biology
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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

09.09.2010

Herausgeber

Joanna M. Bridger + weitere

Verlag

Humana Press

Seitenzahl

451

Maße (L/B/H)

26/18,3/3,5 cm

Gewicht

1051 g

Auflage

2010

Sprache

Englisch

ISBN

978-1-60761-788-4

Beschreibung

Rezension

From the reviews:

“Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. … Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. … I would suggest this is for microbiological libraries … .” (Jonathan Porter, Microbiology Today, November, 2011)

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

09.09.2010

Herausgeber

Verlag

Humana Press

Seitenzahl

451

Maße (L/B/H)

26/18,3/3,5 cm

Gewicht

1051 g

Auflage

2010

Sprache

Englisch

ISBN

978-1-60761-788-4

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Fluorescence in situ Hybridization (FISH)
  • Produktbild: Fluorescence in situ Hybridization (FISH)
  • Part I: Core Techniques

    1. Fluorescence in situ Hybridization (FISH), Basic Principles, and Methodology
    Elisa Garimberti and Sabrina Tosi

    2. Fluorescence in situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibers
    Lauren S. Elcock and Joanna M. Bridger

    3. Detection of Nascent RNA Transcripts by Fluorescence in situ Hybridization
    Jill M. Brown and Veronica J. Buckle

    4. Fluorescence in situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays
    Brenda M. Summersgill and Janet M. Shipley

    5. Fluorescence in situ Hybridization (FISH) on Tissue Cryosections
    Irina Solovei

    6. Multiplex Fluorescence in situ Hybridization (M-FISH)
    Rhona Anderson

    7. Optical Mapping of Protein-DNA Complexes on Chromatin Fibers
    Beth Sullivan

    8. 3D-FISH on Cultured Cells Combined with Immunostaining
    Irina Solovei and Marion Cremer

    Part II: Technical Advancements and Novel Adaptations

    9. The Comet-FISH Assay for the Analysis of DNA Damage and Repair
    Graciela Spivak

    10. Direct in situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes
    Laurent A. Bentolila

    11. LNA-FISH for Detection of microRNAs in Frozen Sections
    Asli N. Silahtaroglu

    12. Chromosome Orientation Fluorescence in situ Hybridization (CO-FISH) or Strand-Specific FISH
    Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, and Edwin H. Goodwin

    13. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells
    Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stei, Carmen Boxler, Patrick Müller, Andriy Mokhir, Roland Krämer, Christoph Cremer, and Michael Hausmann

    14. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation
    Daniela Moralli and Zoia L. Monaco

    15. CryoFISH: Fluorescence in situ Hybridization on Ultrathin Cryosections
    Sheila Q. Xie, Liron-Mark Lavitas, and Ana Pombo

    16. Characterization ofChromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)
    Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, and Nadezda Kosyakova

    17. Visualizing Nucleic Acids in Living Cells by Fluorescence in vivo Hybridization
    Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, and Roeland W. Dirks

    Part III: Translational FISH: Applications for Human Genetics and Medicine

    18. Quality Control in FISH as Part of a Laboratory’s Quality Management System
    Ros Hastings

    19. FlashFISH: “Same Day” Prenatal Diagnosis of Common Chromosomal Aneuploidies
    Sherry Ho and Mahesh A. Choolani

    20. FISH for Pre-Implantation Genetic Diagnosis
    Paul N. Scriven and Caroline Mackie Ogilvie

    21. PNA-FISH on Human Sperm
    Franck Pellestor, Cécile Monzo, and Samir Hamamah

    22. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism
    Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, and Thomas Liehr

    23. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)
    Agata Kowalska, Eva Bozsaky, and Peter F. Ambros

    24. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material
    Soo-Yong Tan and Goran Mattsson

    25. Fluorescence in situ Hybridization on 3D Cultures of Tumor Cells
    Karen J. Meaburn

    26. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization in situ (SUSHI) in HIV-1 Disease Monitoring
    Bruce Patterson

    Part IV: Protocols for Model Organisms

    27. Detection of Prokaryotic Cells with Fluorescence in situ Hybridization
    Katrin Zwirglmaier

    28. FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast
    Harry Scherthan and Josef Loidl

    29. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata
    Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, and Matty Knight

    30. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic