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Produktbild: Protocols in Biochemistry and Clinical Biochemistry
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Protocols in Biochemistry and Clinical Biochemistry

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

22.10.2024

Verlag

Elsevier Science & Technology

Seitenzahl

264

Maße (L/B/H)

23,4/18,8/1,5 cm

Gewicht

499 g

Auflage

2nd edition

Sprache

Englisch

ISBN

978-0-443-13945-1

Beschreibung

Rezension

"...provides an encyclopedic description of about 100 different laboratory techniques for analyzing biomolecules including lipids, carbohydrates, proteins, enzymes, oligonucleotides, vitamins, and does so in a single, compact volume. The purpose of the book is to offer clear hands-on instruction on biochemistry methods and protocols. The book s audience is the students, postdoctoral trainees, technicians, and lead scientists who work day-to-day at the benches of hospital and clinical labs; academic research laboratories; industrial research and development, quality assurance, and quality control labs; and environmental monitoring and waste treatment facilities.... [It] contains nearly double the content of the previous edition, which was published in 2000.... In this spectrum, this book occupies an important niche as a broadly applicable, yet compact and straightforward, resource that should be of great utility to scientists of all types who find themselves needing to analyze biologically derived or clinical materials,... it is highly recommended." ©Doody's Review Service, 2025, Peter Kennelly, PhD (Virginia Tech)

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

22.10.2024

Verlag

Elsevier Science & Technology

Seitenzahl

264

Maße (L/B/H)

23,4/18,8/1,5 cm

Gewicht

499 g

Auflage

2nd edition

Sprache

Englisch

ISBN

978-0-443-13945-1

EU-Ansprechpartner

Zeitfracht Medien GmbH
Ferdinand-Jühlke-Straße 7
99095 Erfurt
DE
produktsicherheit@zeitfracht.de

Herstelleradresse

Elsevier Science & Technology
London Wall 125
EC2Y 5AS London
GB
tradeorders@elsevier.com

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  • Produktbild: Protocols in Biochemistry and Clinical Biochemistry

  • Solutions, Acids and Bases
    1. Preparation of Solutions
    2. Acid and Base

    Carbohydrates
    3. Determination of the presence of carbohydrates in the given sample by Molisch’s test
    4. Determination of the presence of reducing carbohydrates in the given sample by Picric acid test
    5. Determination of the presence of reducing carbohydrates in the given sample by Fehling’s test
    6. Determination of the presence of reducing carbohydrates in the given sample by Benedict’s test
    7. Determination of the presence of reducing carbohydrates in the given sample by Tommer’s test
    8. Determination of the presence of reducing carbohydrates in the given sample by Nylander’s test
    9. Distinguish between monosaccharides and reducing disaccharides by Barfoed’s test.
    10. Distinguish between aldose and ketose by Seliwanoff’s test.
    11. Determination of pentose sugar by Bial’s test
    12. Determination of the presence of galactose in a test sample by the Mucic acid test
    13. Differentiation between the presence of ketohexose or aldohexose in the test sample by Foulger'stest
    14. Determination of the presence of reducing carbohydrates in the given sample by Fearon’s methylamine test
    15. Confirmation of the presence of reducing carbohydrates in the given sample by osazone formation test
    16. Determination of the presence of polysaccharides in the given sample by an iodine test
    17. Quantitative estimation of carbohydrates in the given sample by anthrone test.
    18. Quantitative estimation of reducing carbohydrates in the given sample by 3,5-dinitro salicylic acid (DNSA) test.
    19. Extraction and estimation of glycogen from the liver and muscle of the well-fed and starved rat.
    20. Separation and Identification of sugars present in fruit juices using thin-layer chromatography (TLC).
    21. Extraction and analysis of soluble carbohydrates from plants.

    Lipids
    22. Solubility test for lipids
    23. Emulsion test for lipids.
    24. To determine the degree of unsaturation of fatty acids
    25. Determination of the saponification value of the given fat/ oil sample
    26. To determine the fatty acid value of fat
    27. Acrolein test for the presence of glycerol
    28. Libermann - Buchard test for detection of cholesterol.
    29. Extraction of lipid from leaves (Bligh and Dyer’s method and single extraction method).
    30. Extraction of lipid from egg yolk and estimation of phosphorous content in it
    31. Extraction of lipid from tissues (Folch method).
    32. Identification of lipids by TLC.
    33. Lipid per oxidation measurement by Thiobarbituric acid (TBA) assay.

    Proteins
    34. Isolation of protein from animal cells/tissue using RIPA buffer
    35. Qualitative test for Proteins and amino acids
    36. Estimation of proteins by Biuret method
    37. Estimation of proteins by Bradford method
    38. Estimation of proteins by Bicinchoninic acid (BCA) method
    39. Estimation of proteins by Folin-Lowry method
    40. Estimation of proteins by UV spectrophotometric method
    41. Protein separation by gel electrophoresis under denaturing condition (SDS-PAGE)
    42. Separation of amino acids by paper chromatography.

    Minerals
    43. Estimation of the Calcium in water or milk by EDTA titrimetric method
    44. Estimation of the Calcium in serum by O-Cresolpthaleincomplexon method (colorimetric)
    45. Estimation of the inorganic phosphorus by colorimetric method
    46. Estimation of the Iron by thiocyanate colorimetric method
    47. Estimation of the Chloride by Titration method (Mohr Argentometric method)

    Vitamin
    48. Estimation of Vitamin A by Trichloroacetic acid (modified Carr-Price method)
    49. Estimation of the Tocopherol (Vitamin E) by colorimeter (Baker and Frank method)
    50. Estimation of the Thiamine (Vitamin B1) by Thiochrome technique
    51. Estimation of the Ascorbic acid (Vitamin C) by 2,6-dichlorophenol indophenol (DCPIP) method (Titrimetric)
    52. Estimation of the Ascorbic acid (Vitamin C) by 2, 4 Dinitrophenylhydrazine method (Colorimetric)

    Enzymes
    53. To study the effect of pH and temperature on the action of salivary amylase.
    54. Determination of the activity of amylase enzyme (crude) extracted from sweet potato.
    55. Determination of the specific activity of the amylase enzyme.
    56. To determine Vmax and Km in of the amylase enzyme.

    Nucleic acid
    57. Determination of the amount of DNA present in the solution by diphenylamine (DPA) method
    58. Determination of the amount of RNA present in the solution by orcinol method.
    59. Isolation of plasmid DNA using the alkaline lysis method.
    60. Preparation of plasmid DNA using boiling lysis method.
    61. Plasmid DNA purification using polyethylene glycol.
    62. Isolation of genomic DNA of the unknown bacterial strain.
    63. Isolation of DNA from plant tissue.
    64. Isolation of DNA from a eukaryotic cell using proteinase K and phenol.
    65. Isolation of DNA from a eukaryotic cell.
    66. Separation of bands of DNA by agarose gel electrophoresis.
    67. SOUTHERN TRANSFER AND HYBRIDIZATION: To perform Southern blotting of the genomic DNA.
    68. Isolation of RNA from animal cells.
    69. NORTHERN BLOTTING.

    Clinical Biochemistry
    70. Determination of Serum Urea [Diacetyl Monoxime (DAM) Method],
    71. Estimation of Uric Acid in Serum Sample (Uricase Method),
    72. Estimation of Creatinine in Serum Samples (Jaffe’s Method),
    73. Determination of Serum Glutamate Pyruvate Transaminase (SGPT) Activity,
    74. Determination of Serum Glutamate Oxaloacetate Transaminase (SGOT) Activity,
    75. Colorimetric Estimation of Alkaline Phosphatase (ALP) From Serum (King and Armstrong),
    76. Estimation of Serum Albumin by the Dye-Binding Method (Bromocresol Green Method),
    77. Estimation of Blood Glucose by the Glucose Oxidase Method,
    78. Estimation of Glucose in Urine by Benedict Method,
    79. Estimation of Percentage of Glucose (Reducing Sugars) in Urine (by Benedict Titration Method),
    80. Oral Glucose Tolerant Test (OGTT),
    81. Estimation of Total Cholesterol in Serum (Watson Method),
    82. Estimation of Total Cholesterol in Serum (Enzymatic Method),
    83. Estimation of Triglycerides in Serum (Enzymatic Method),
    84. Estimation of Hemoglobin in the Blood (Sahli’s Method).
    85. Determination of serum amylase.
    86. Catalase activity assay.
    87. Superoxide dismutase (SOD) assay.

    Plant Biochemistry
    88. Qualitative analysis of plant secondary metabolites.
    89. Qualitative analysis of photochemical.
    90. Quantitative estimation of secondary metabolites.
    91. Estimation of Chlorophyll contents.
    92. Determination of starch