FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY



The Cell as the Basic Unit of Life



Structure and Function of Cellular Macromolecules



Structure and Functions of a Cell



Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins)



Distributing Proteins in the Cell (Protein Sorting)



Evolution and Diversity of Organisms



 



STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY



Isolation and Purification of Proteins



Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry



Isolation of DNA and RNA



Chromatography and Electrophoresis of Nucleic Acids



Hybridization of Nucleic Acids



Use of Enzymes in the Modification of Nucleic Acids



Polymerase Chain Reaction



DNA Sequencing



Genome Editing



Cloning Procedures



Expression of Recombinant Proteins



Omics technologies



Patch Clamp Method



Cell Cycle Analysis



Microscopic Techniques



Laser Applications



 



KEY TOPICS



Genomics and Functional Genomics



Bioinformatics



Cellular Systems Biology



Protein-Protein and Protein-DNA Interaction



Drug Research



Drug Targeting and Prodrugs



Molecular Diagnostics and Personalized Medicine



Recombinant Antibodies and Phage Display



Transgenic and Gene-Targeted Mice



Plant Biotechnology



Industrial Biocatalysis



 



BIOTECHNOLOGY IN INDUSTRY



The Biotech Industry, Markets, and Opportunities



Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues



Drug Approval in the European Union and United States



Founding a Biotech Company



 



APPENDIX



Further Reading



Abbreviations



Subject Index